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Structured Review

Synthego Inc 5x annealing buffer
5x Annealing Buffer, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/annealing+buffer/buffer/pm41715271-95-0-3
Average 86 stars, based on 1 article reviews
5x annealing buffer - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Isolation:

Article Title: In vitro isolation and enrichment of nucleic acids using site-specific nucleases
Article Snippet: .. Isolation of a Nucleic Acid Target Region Using a Catalytically Dead Type II Cas Protein First, crRNA-tracrRNA duplexes were prepared by mixing 20 μM of crRNA with 10 μM of tracrRNA (IDT) in 1X Synthego annealing buffer, to generate the Cas9 gRNA-Ecoli#1 comprising the target specific sequence according to SEQ ID NO: 6). ..

Sequencing:

Article Title: In vitro isolation and enrichment of nucleic acids using site-specific nucleases
Article Snippet: .. Isolation of a Nucleic Acid Target Region Using a Catalytically Dead Type II Cas Protein First, crRNA-tracrRNA duplexes were prepared by mixing 20 μM of crRNA with 10 μM of tracrRNA (IDT) in 1X Synthego annealing buffer, to generate the Cas9 gRNA-Ecoli#1 comprising the target specific sequence according to SEQ ID NO: 6). ..

Article Title: Dynamics and competition of CRISPR–Cas9 ribonucleoproteins and AAV donor-mediated NHEJ, MMEJ and HDR editing
Article Snippet: .. To prepare for 30 μM annealed gRNA, crRNA and tracrRNA were mixed in a 2:1 ratio and diluted in an annealing buffer provided by Synthego, followed by treating at 78°C for 10 min, 37°C for 30 min, and room temperature (23°C) for 15 min. Alt-R ® SpCas9 Nuclease V3 protein, which contains a nuclear localization sequence, was purchased from IDT. ..

Concentration Assay:

Article Title: OliTag-seq enhances in cellulo detection of CRISPR-Cas9 off-targets.
Article Snippet: .. Annealed gRNAs were prepared at a working concentration of 30 μMbymixing crRNA and tracrRNA in a 2:1 ratio, followed by dilution in 5× annealing buffer (Synthego). ..



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